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Objective:To explore the application effect of self-management ability-oriented focus solution model in children with nephrotic syndrome, and to observe the optimal value of self-management and coping style for children with nephrotic syndrome.Methods:This was a class trial study. A total of 96 children with nephrotic syndrome in Children′s Hospital of Nanjing Medical Universityfrom March 2018 to April 2020 were selected as the research object by convenient sampling method, 48 cases of children from March 2018 to February 2019 for the control group, using conventional psychological counseling, 48 cases of children from March 2019 to April 2020 for the observation group, with self management ability oriented focus solution mode. The self-management and response changes after 2 months of intervention were compared between the two groups.Results:The scores of treatment management, psychological management, diet management and social activity inself-management behavior after intervention were (32.79 ± 2.94), (19.43 ± 1.76), (22.34 ± 1.64), (39.79 ± 2.97) points in the observation group, and (27.42 ± 3.25), (16.79 ± 1.52), (18.46 ± 1.58), (35.28 ± 2.64) points in the control group, the differences were statistically significant ( t values were 7.86-11.80, all P<0.05). The scores of escape, acceptance, negative emotional response, and total score after intervention were (12.41 ± 2.15), (20.41 ± 1.85), (25.89 ± 1.68), (99.37 ± 8.94) points in the observation group, and (11.22 ± 3.04), (19.30 ± 2.09), (24.80 ± 2.10), (95.12 ± 9.02) points in the control group, the differences were statistically significant ( t values were 2.21-2.81, all P<0.05). Conclusions:The self-management-oriented focus solution model for children with nephrotic syndrome can promote the improvement of self-management behavior, and promote the improvement of coping methods in terms of avoidance, acceptance and negative emotional response, which has reference and complementary value for the care of children with nephrotic syndrome.
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Objective To construct the recombinant streptolysin O antigen(SLO) prokaryotic expression plasmid and establish its best expression condition in Escherichia coli .Methods The DNA fragment encoding SLO was amplified from streptococcal ge-nomic DNA template by PCR ,and then incorporated into pET-32a(+ ) vector to construct pET-32a(+ )-SLO recombinant plas-mid .pET-32a(+ )-SLO was transformed into Escherichia coli BL21(DE3) and SLO protein was expressed and purified by isopro-pyl-β-D-thiogalactoside(IPTG)-induction and auto-induction ,respectively .Results The results of DNA electrophoresis and DNA sequencing showed that pET-32a(+ )-SLO recombinant plasmid was constructed successfully .When IPTG at different concentra-tion was used ,SLO expressed as inclusion body and its expression efficiency was low .Under auto-induction condition ,SLO ex-pressed as partly soluble manner ,and its expression efficiency increased .Conclusion The prokaryotic expression plasmid pET-32a (+ )-SLO is constructed successfully and the best condition for SLO expression and purification from Escherichia coli culture is es-tablished ,which lay the foundation for further basic and clinical application research with SLO .